recombinant human il 18  (R&D Systems)

Journal: iScience

Article Title: Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cells

doi: 10.1016/j.isci.2026.115327

Figure Lengend Snippet: TNFR1-and HER2-specific bispecific bivalent ICMs (2 + 2) trigger reporter cell activity (A) HEK-Blue TNF reporter cells were incubated with two fixed concentrations of 10 nM and 1 nM of bsAbs, as well as 0.1 nM and 0.01 nM of (rh) TNF as a positive control and 0.1 nM of (rh) IL-18 as a negative control. After 24 h of incubation, secreted embryonic alkaline phosphatase activity was measured via OD 640 . Reporter activity was normalized to (rh) TNF signal. (B) Dose-titration of agonistic bsAbs in HEK-Blue TNF reporter cells. Mean values ±SEM of three independent experiments are shown. ∗∗∗∗ p < 0.0001,∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05 calculated by utilizing one-way ANOVA analyses and Bonferroni test.

Article Snippet: Incubation of the HEK-Blue TNF cells with 0.1 nM and 0.01 nM of TNF and IL-18 (R&D Systems, 9124-IL) were used as positive and negative control.

Techniques: Activity Assay, Incubation, Positive Control, Negative Control, Titration